Search

Your search keyword '"Langhans, Markus"' showing total 199 results
Start Over Author "Langhans, Markus"
199 results on '"Langhans, Markus"'

2. Nanomechanical subsurface characterisation of cellulosic fibres

Author

Auernhammer, Julia, Langhans, Markus, Schäfer, Jan-Lukas, Keil, Tom, Meckel, Tobias, Biesalski, Markus, and Stark, Robert W.

Subjects
Physics - Applied Physics, Physics - Chemical Physics
Abstract

The mechanical properties of single fibres are highly important in the paper production process to produce and adjust properties for the favoured fields of application. The description of mechanical properties is usually characterised via linearized assumptions and is not resolved locally or spatially in three dimensions. In tensile tests or nanoindentation experiments on cellulosic fibres, only one mechanical parameter, such as elastic modulus or hardness, is usually obtained. To obtain a more detailed mechanical picture of the fibre, it is crucial to determine mechanical properties in depth. To this end, we discuss an atomic force microscopy-based approach to examine the local stiffness as a function of indentation depth via static force-distance curves. This method has been applied to linter fibres (extracted from a finished paper sheet) as well as to natural raw cotton fibres to better understand the influence of the pulp treatment process in paper production on the mechanical properties. Both types of fibres were characterised in dry and wet conditions with respect to alterations in their mechanical properties. Subsurface imaging revealed which wall in the fibre structure protects the fibre against mechanical loading. Via a combined 3D display, a spatially resolved mechanical map of the fibre interior near the surface can be established. Additionally, we labelled fibres with carbohydrate binding modules tagged with fluorescent proteins to compare the AFM results with fluorescence confocal laser scanning microscopy imaging. Nanomechanical subsurface imaging is thus a tool to better understand the mechanical behaviour of cellulosic fibres, which have a complex, hierarchical structure.

Published
2021

6. Modulation of Differentiation and Bone Resorbing Activity of Human (Pre-) Osteoclasts After X-Ray Exposure

Author

Eckert, Denise, primary, Rapp, Felicitas, additional, Tsedeke, Ayele Taddese, additional, Kraft, Daniela, additional, Wente, Isabell, additional, Molendowska, Jessica, additional, Basheer, Sidra, additional, Langhans, Markus, additional, Meckel, Tobias, additional, Friedrich, Thomas, additional, Donaubauer, Anna-Jasmina, additional, Becker, Ina, additional, Frey, Benjamin, additional, and Fournier, Claudia, additional

Published
2022
Full Text
View/download PDF

12. Carbohydrate binding module-fused antibodies improve the performance of cellulose-based lateral flow immunoassays

Author

Elter, Adrian, primary, Bock, Tina, additional, Spiehl, Dieter, additional, Russo, Giulio, additional, Hinz, Steffen C., additional, Bitsch, Sebastian, additional, Baum, Eva, additional, Langhans, Markus, additional, Meckel, Tobias, additional, Dörsam, Edgar, additional, Kolmar, Harald, additional, and Schwall, Gerhard, additional

Published
2021
Full Text
View/download PDF

14. Combination of hydrophobicity and codon usage bias determines sorting of model K+ channel protein to either mitochondria or endoplasmic reticulum.

Author

Engel, Anja J., Paech, Steffen, Langhans, Markus, van Etten, James L., Moroni, Anna, Thiel, Gerhard, and Rauh, Oliver

Subjects
RIBOSOMES, MITOCHONDRIAL proteins, ENDOPLASMIC reticulum, TRANSMEMBRANE domains, MITOCHONDRIA, PROTEIN folding, GENETIC code
Abstract

When the K+ channel‐like protein Kesv from Ectocarpus siliculosus virus 1 is heterologously expressed in mammalian cells, it is sorted to the mitochondria. This targeting can be redirected to the endoplasmic reticulum (ER) by altering the codon usage in distinct regions of the gene or by inserting a triplet of hydrophobic amino acids (AAs) into the protein's C‐terminal transmembrane domain (ct‐TMD). Systematic variations in the flavor of the inserted AAs and/or its codon usage show that a positive charge in the inserted AA triplet alone serves as strong signal for mitochondria sorting. In cases of neutral AA triplets, mitochondria sorting are favored by a combination of hydrophilic AAs and rarely used codons; sorting to the ER exhibits the inverse dependency. This propensity for ER sorting is particularly high when a common codon follows a rarer one in the AA triplet; mitochondria sorting in contrast is supported by codon uniformity. Since parameters like positive charge, hydrophobic AAs, and common codons are known to facilitate elongation of nascent proteins in the ribosome the data suggest a mechanism in which local changes in elongation velocity and co‐translational folding in the ct‐TMD influence intracellular protein sorting. [ABSTRACT FROM AUTHOR]

Published
2023
Full Text
View/download PDF

17. ROS- and Radiation Source-Dependent Modulation of Leukocyte Adhesion to Primary Microvascular Endothelial Cells

Author

Eckert, Denise, Rapp, Felicitas, Tsedeke, Ayele T., Molendowska, Jessica, Lehn, Robert, Langhans, Markus, Fournier, Claudia, R��del, Franz, and Hehlgans, Stephanie

Subjects
NF-E2-Related Factor 2, leukocytes, QH301-705.5, Models, Biological, Article, shear stress, ddc:570, Cell Adhesion, Humans, RNA, Messenger, Biology (General), low-dose irradiation, Cells, Cultured, X-Rays, Dose-Response Relationship, Radiation, Carbon, endothelial cells, Oxidative Stress, adhesion, Gene Expression Regulation, inflammation, Microvessels, Reactive Oxygen Species, Cell Adhesion Molecules, Oxidation-Reduction
Abstract

Cells 11(1), 72 (2022). doi:10.3390/cells11010072, Published by MDPI, Basel

Published
2022

25. Codon Bias Can Determine Sorting of a Potassium Channel Protein

Author

Engel, Anja J., Kithil, Marina, Langhans, Markus, Rauh, Oliver, Cartolano, Matea, Etten, James L. Van, Moroni, Anna, and Thiel, Gerhard

Subjects
Protein Transport, dual sorting, Potassium Channels, codon usage, QH301-705.5, Genetic Code, Protein Biosynthesis, Humans, membrane protein sorting, Biology (General), Article, effect of synonymous codon exchange
Abstract

Due to the redundancy of the genetic code most amino acids are encoded by multiple synonymous codons. It has been proposed that a biased frequency of synonymous codons can affect the function of proteins by modulating distinct steps in transcription, translation and folding. Here, we use two similar prototype K+ channels as model systems to examine whether codon choice has an impact on protein sorting. By monitoring transient expression of GFP-tagged channels in mammalian cells, we find that one of the two channels is sorted in a codon and cell cycle-dependent manner either to mitochondria or the secretory pathway. The data establish that a gene with either rare or frequent codons serves, together with a cell-state-dependent decoding mechanism, as a secondary code for sorting intracellular membrane proteins.

Published
2021
Full Text
View/download PDF

38. Synthesis and Biological Properties of Novel Brefeldin A Analogues

Author

Seehafer, Kai, primary, Rominger, Frank, additional, Helmchen, Günter, additional, Langhans, Markus, additional, Robinson, David G., additional, Özata, Başak, additional, Brügger, Britta, additional, Strating, Jeroen R. P. M., additional, van Kuppeveld, Frank J. M., additional, and Klein, Christian D., additional

Published
2013
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results